Cryopreservation of persimmon (Diospyros kaki Thunb.) by vitrification of dormant shoot tips

Citation
T. Matsumoto et al., Cryopreservation of persimmon (Diospyros kaki Thunb.) by vitrification of dormant shoot tips, PL CELL REP, 20(5), 2001, pp. 398-402
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
20
Issue
5
Year of publication
2001
Pages
398 - 402
Database
ISI
SICI code
0721-7714(200107)20:5<398:COP(KT>2.0.ZU;2-C
Abstract
Shoot tips excised from dormant axillary buds of persimmon (Diospyros kaki Thunb.) were cryopreserved by vitrification. These excised shoot tips were dehydrated in a highly concentrated vitrification solution for 20 min at 25 degreesC and then plunged directly into liquid nitrogen. After rapid warmi ng in water at 40 degreesC, the shoot tips were rinsed in a 1.2 M sucrose s olution for 20 min and then plated on a solidified culture medium. Successf ully vitrified shoot tips resumed growth within 10 days of plating and deve loped shoots within 3 weeks without intermediary callus formation. This sim ple protocol was successfully applied to the 16 cultivars found in the temp erate zone. The average rate of shoot formation was 89%. Even the subtropic al species of Diospyros demonstrated a very high recovery growth when the s hoot tips had been previously osmoprotected with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min following sucrose preculture. Little or no co ntamination occurred in the cryopreserved shoot tips excised from sterilize d winter axillary buds. Thus, this simple and reliable vitrification protoc ol using dormant shoot tips appears to be promising as a routine method for the longterm conservation of Diospyros germplasm of both temperate and sub tropical origins.