Identification of novel inhibitors of calling and in vitro [C-14]acetate incorporation by pheromone glands of Plodia interpunctella

Citation
A. Hirashima et al., Identification of novel inhibitors of calling and in vitro [C-14]acetate incorporation by pheromone glands of Plodia interpunctella, PEST MAN SC, 57(8), 2001, pp. 713-720
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Entomology/Pest Control
Journal title
PEST MANAGEMENT SCIENCE
ISSN journal
1526-498X → ACNP
Volume
57
Issue
8
Year of publication
2001
Pages
713 - 720
Database
ISI
SICI code
1526-498X(200108)57:8<713:IONIOC>2.0.ZU;2-M
Abstract
Some octopamine agonists were found to suppress in vitro biosynthesis of th e calling pheromone of the Indian meal moth, Plodia interpunctella. Isolate d pheromone-gland preparations incorporated Sodium [C-14]acetate at a linea r rate for 3h when incubated with the pheromone biosynthesis activating neu ropeptide (PBAN). This incorporation was dependent on the dose of PBAN (up to 0.5 muM). Thin-layer chromatography of a pheromone-gland extract reveale d quantitative incorporation of radioactivity into a product exhibiting the same mobility as (Z,E)-9,12-tetradecadienyl acetate, the main component of the calling pheromone of P interpunctella. Twenty-seven octopamine agonist s were initially screened using a calling behaviour bioassay of female P in terpunctella. Four derivatives with activity in the nanomolar range were id entified which were, in order of decreasing pheromonostatic activity: 2-(2, 6-diethylphenylimino)thiazolidine > 2-(2,6-diethylphenylimino)oxazolidine > 2-(2,6-dimethylphenylimino)thiazolidine > 2-(2-ethylphenylimino)oxazolidin e. These compounds also showed in vitro inhibitory activity in intracellula r de novo pheromone biosynthesis. The results of the present study indicate that these derivatives could provide useful information in the characteriz ation and differentiation of octopaminergic receptor types and subtypes. (C ) 2001 Society of Chemical Industry.