Protein tyrosine kinase expression in the porcine ovary

Citation
Y. Okamura et al., Protein tyrosine kinase expression in the porcine ovary, MOL HUM REP, 7(8), 2001, pp. 723-729
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR HUMAN REPRODUCTION
ISSN journal
1360-9947 → ACNP
Volume
7
Issue
8
Year of publication
2001
Pages
723 - 729
Database
ISI
SICI code
1360-9947(200108)7:8<723:PTKEIT>2.0.ZU;2-R
Abstract
Various growth factor receptors contain intrinsic tyrosine kinase activity, indicating that protein tyrosine kinases (PTK) play an important role in s ignal transduction pathways for cell proliferation and differentiation. To identify oocyte-derived factors which control follicle cells as well as ooc yte-controlling factors produced by follicle cells, we examined the express ion of genes which contain the PTK domain in the porcine ovary, using a pol ymerase chain reaction-based amplification technique with degenerate oligon ucleotide primers that are specific to the PTK domain. Clones for the porci ne homologues of platelet-derived growth factor receptor alpha (PDGFRalpha) and of insulin-like growth factor-I receptor (IGF-IR) were found during fo llicle growth both in oocytes and follicle cells. Clones for the porcine ho mologues of focal adhesion kinase (FAK), of c-kit and of fms-like tyrosine kinase (FLT)-3 were found only in oocytes. Moreover, after 24 h of in-vitro maturation of the cumulus-oocyte complexes, clones for the porcine homolog ues of FLT-1, of FLT-4, of Tie2 and of RYK in oocytes were observed. Immuno histochemical studies revealed the existence of PDGFR alpha, platelet-deriv ed growth factor alpha (PDGFralpha), FAK and FLT3 in oocytes at various sta ges of folliculogenesis. These results suggest that fluctuations in the exp ression of these PTK genes may be involved in follicle growth and maturatio n.