A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation

Citation
M. Matsushita et al., A high-efficiency protein transduction system demonstrating the role of PKA in long-lasting long-term potentiation, J NEUROSC, 21(16), 2001, pp. 6000-6007
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE
ISSN journal
0270-6474 → ACNP
Volume
21
Issue
16
Year of publication
2001
Pages
6000 - 6007
Database
ISI
SICI code
0270-6474(20010815)21:16<6000:AHPTSD>2.0.ZU;2-7
Abstract
Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that protein transduction by 11 arginine (11R) is an efficient method of delive ring proteins into the neurons of brain slices. Here, we demonstrate that P KA inhibitory peptide, fused with 11R and nuclear localization signal, deli vers the peptide exclusively into the nuclear compartment of neurons in bra in slices. This inhibitory peptide blocked both cAMP responsive element-bin ding protein phosphorylation and long-lasting long-term potentiation (LTP) induction, but not early LTP. These results highlight transduction of prote ins and peptides into specific neuronal subcellular compartments in brain s lices as a powerful tool for studying neuronal plasticity.