Th1/Th2 cytokine profiles and their relationship to clinical features in patients with chronic hepatitis C virus infection

Citation
S. Sobue et al., Th1/Th2 cytokine profiles and their relationship to clinical features in patients with chronic hepatitis C virus infection, J GASTRO, 36(8), 2001, pp. 544-551
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
JOURNAL OF GASTROENTEROLOGY
ISSN journal
0944-1174 → ACNP
Volume
36
Issue
8
Year of publication
2001
Pages
544 - 551
Database
ISI
SICI code
0944-1174(200108)36:8<544:TCPATR>2.0.ZU;2-N
Abstract
Purpose. An imbalance in helper T-cell type 1 (Th1) and type 2 (Th2) cytoki nes is suggested to play an important role in the pathogenesis of chronic v iral infections, but this issue is not resolved in patients with hepatitis C virus (HCV) infection. The aim of this study was to clarify the relations hip between the balance of Th1 and Th2 cytokines and liver damage. Methods. We investigated cytokine levels in the peripheral blood and liver tissue o f patients with chronic HCV infection (n = 59) by three different methods; we used flow cytometry to detect intracellular cytokines, and we measured c ytokine titers in sera and in the supernatants of lymphocyte cultures with enzyme-linked immunosorbent assays (ELISAs). Results. In both CD4+ and CD8 cells, interferon (IFN) gamma -producing cell populations increased, while there was no difference in interleukin (IL)-10 production, indicating a sh ift to a Th1 cytokine profile with the progression of liver disease. With r espect to the ratio of IFN-gamma to IL-10, a correlation was found in CD4cells between peripheral blood and liver tissue (r = 0.98; P = 0.0011). Th1 cytokine was predominant in intrahepatic CD4+ cells, while it was predomin ant in peripheral blood CD8+ cells. Conclusions. These findings indicate a correlation between dominant Th1 response and disease activity and progress ion. In addition, we suggest that intrahepatic CD4+ T cells play a pathogen etic role in the hepatic injury of HCV infection.