Electrochromatographic characterization of etched chemically-modified capillaries with small synthetic peptides

Citation
Mt. Matyska et al., Electrochromatographic characterization of etched chemically-modified capillaries with small synthetic peptides, J CHROMAT A, 924(1-2), 2001, pp. 211-221
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
924
Issue
1-2
Year of publication
2001
Pages
211 - 221
Database
ISI
SICI code
0021-9673(20010727)924:1-2<211:ECOECC>2.0.ZU;2-N
Abstract
In this investigation, various capillary electrochromatographic (CEC) exper iments have been employed to characterize the properties of etched, chemica lly-modified surfaces of open tubular capillary columns with peptides as so lute probes and under conditions of variable voltage, temperature and solve nt composition. The separation performance of etched capillaries with eithe r n-octadecyl or liquid crystal moieties derived from a cholesterol phase b onded to the surface were compared. With the liquid crystal bonded species, interesting and significantly different variations in retention behavior o f peptides are obtained compared to those observed with the corresponding n -octadecyl modified surfaces by changes in temperature, solvent composition and field strength. These peptide separations illustrate the usefulness of this CEC approach for practical applications, where both the retention cha racteristics of the charged analytes as well as the selectivity differences due to the surface properties of the etched chemically-modified surfaces o f open tubular capillary columns can be rationally modulated. As in HPLC, a ppropriate choice of CEC experimental variables, including the chemical pro perties of the immobilized ligand(s), represents a powerful tool for optimi zing resolution. (C) 2001 Elsevier Science B.V. All rights reserved.