Purpose. To determine whether an intraoperative single dose of dexamethason
e, diclofenac, ethylenediaminetetraacetic acid (EDTA), a combination of EDT
A and RGD peptide (arginine-glycin-aspartic acid sequence), or mitomycin-C
(MMC) is a pharmacological means of preventing or reducing the development
of posterior capsule opacification (PCO).
Setting. Department of Ophthalmology, Celal Bayar University, School of Med
icine, Manisa, and Department of Pathology, Dokur Eylul University, School
of Medicine, izmir, Turkey.
Methods: Fifty-four rabbits were randomly divided into 6 groups. Dexamethas
one (4 mg/cc), diclofenac (2.5 mg/cc), EDTA (8 mg/cc), a combination of EDT
A and RGD peptide (2.5 mg/cc), or MMC (0.04 mg/cc) was given, 0.1 cc by hyd
rodissection and 0.9 cc into the capsular bag after phacoemulsification. Th
e sixth group served as a control group. After 3 months, the PCO was graded
clinically and the proliferation of lens epithelial cells (LECs) was evalu
Results: The drugs were significantly effective in preventing PCO compared
with the control (P < .005). Dexamethasone had a weaker effect than the oth
er drugs. In histological analysis, although monolayer LECs in the dexameth
asone and diclofenac groups were observed, there was no proliferative activ
ity on the posterior capsules in the EDTA, EDTA+RGD, and MMC groups in cont
rast to the multilayer cells in the control.
Conclusions: Intraoperative single-dose application of EDTA, EDTA+RGD pepti
de combination, and MMC significantly prevented the development of PCO in r
abbit eyes. Diclofenac was less effective but also reduced PCO. Although de
xamethasone did not prevent the proliferation of LECs, it decreased PCO cli
nically. (C) 2001 ASCRS and ESCRS.