Migration of retinal pigment epithelial cells in vitro modulated by monocyte chemotactic protein-1: enhancement and inhibition

Citation
Qh. Han et al., Migration of retinal pigment epithelial cells in vitro modulated by monocyte chemotactic protein-1: enhancement and inhibition, GR ARCH CL, 239(7), 2001, pp. 531-538
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Optalmology
Journal title
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
ISSN journal
0721-832X → ACNP
Volume
239
Issue
7
Year of publication
2001
Pages
531 - 538
Database
ISI
SICI code
0721-832X(200107)239:7<531:MORPEC>2.0.ZU;2-3
Abstract
Background: The migration of retinal pigment epithelial (RPE) cells is an i nitial step in the development of proliferative vitreoretinopathy (PVR). Th is in vitro study was carried out to investigate the effects of monocyte ch emotactic protein-1 (MCP-1) on the migration and proliferation of RPE cells . Methods: We used an in vitro wound healing model in which a small area of a confluent monolayer of human RPE (HRPE) cells was denuded with a razor bla de. The cultures were subsequently incubated with MCP-1, IL-1 beta, TNF-alp ha, or combinations thereof. Neutralizing IgG, of antihuman MCP-1, dexameth asone (DEX) or daunorubicin were also added to the cultures to test their i nhibitory effects on migration of RPE cells. HRPE migration was measured as the number of cells that entered the denuded area. The effect of MCP-1 on proliferation of HRPE cells was examined by MTT assay. Results: MCP-1 stimulated HRPE cell migration in a dose-dependent manner. I L-1 beta or TNF-alpha slightly stimulated HRPE cell migration, but adding a nti-MCP-1 IgG1 significantly reduced this effect. MCP-1-induced migration c ould be inhibited by DEX but not by daunorubicin. MCP-1 did not show a sign ificant effect on HRPE cell proliferation. Conclusion: MCP-1 stimulates HRPE cell migration, suggesting that this chem okine regulates the development of PVR at the initial stage. The migration of HRPE cells induced by IL-1 beta and TNF-alpha may be associated with the MCP-1 that HRPE cells secretes under the stimulation of these two cytokine s. The knowledge that MCP-1-induced migration of HRPE cells is inhibited by DEX may be useful in devising an effective treatment for PVR.