Qh. Han et al., Migration of retinal pigment epithelial cells in vitro modulated by monocyte chemotactic protein-1: enhancement and inhibition, GR ARCH CL, 239(7), 2001, pp. 531-538
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Optalmology
Journal title
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
Background: The migration of retinal pigment epithelial (RPE) cells is an i
nitial step in the development of proliferative vitreoretinopathy (PVR). Th
is in vitro study was carried out to investigate the effects of monocyte ch
emotactic protein-1 (MCP-1) on the migration and proliferation of RPE cells
.
Methods: We used an in vitro wound healing model in which a small area of a
confluent monolayer of human RPE (HRPE) cells was denuded with a razor bla
de. The cultures were subsequently incubated with MCP-1, IL-1 beta, TNF-alp
ha, or combinations thereof. Neutralizing IgG, of antihuman MCP-1, dexameth
asone (DEX) or daunorubicin were also added to the cultures to test their i
nhibitory effects on migration of RPE cells. HRPE migration was measured as
the number of cells that entered the denuded area. The effect of MCP-1 on
proliferation of HRPE cells was examined by MTT assay.
Results: MCP-1 stimulated HRPE cell migration in a dose-dependent manner. I
L-1 beta or TNF-alpha slightly stimulated HRPE cell migration, but adding a
nti-MCP-1 IgG1 significantly reduced this effect. MCP-1-induced migration c
ould be inhibited by DEX but not by daunorubicin. MCP-1 did not show a sign
ificant effect on HRPE cell proliferation.
Conclusion: MCP-1 stimulates HRPE cell migration, suggesting that this chem
okine regulates the development of PVR at the initial stage. The migration
of HRPE cells induced by IL-1 beta and TNF-alpha may be associated with the
MCP-1 that HRPE cells secretes under the stimulation of these two cytokine
s. The knowledge that MCP-1-induced migration of HRPE cells is inhibited by
DEX may be useful in devising an effective treatment for PVR.