Autoimmune responses to mRNA binding proteins p62 and Koc in diverse malignancies

Citation
Jy. Zhang et al., Autoimmune responses to mRNA binding proteins p62 and Koc in diverse malignancies, CLIN IMMUNO, 100(2), 2001, pp. 149-156
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
CLINICAL IMMUNOLOGY
ISSN journal
1521-6616 → ACNP
Volume
100
Issue
2
Year of publication
2001
Pages
149 - 156
Database
ISI
SICI code
1521-6616(200108)100:2<149:ARTMBP>2.0.ZU;2-U
Abstract
Two tumor-associated antigens, p62 and Koc, are insulin-like growth factor II (IGF-II) messenger RNA binding proteins. Autoantibodies to p62 have been detected in cancer sera but have not been reported for Koc. This study det ermined the extent and frequency of autoantibodies to p62 and Koc in divers e malignancies, the epitopes on the antigens, and the presence or absence o f cross-reactive antibodies. Recombinant polypeptides were expressed from f ull-length and partial cDNA constructs and used as antigens in Western blot ting, enzyme-linked immunoassay, and immunoprecipitation. After identifying the epitopes, cross-absorption with recombinant polypeptides was used to d etermine specificity. Sera from 777 patients with 10 different types of mal ignancy were analyzed. Autoantibodies to p62 were found in 11.6% and to Koc in 12.2% and cumulatively to both antigens in 20.5%, with significant diff erence from the control populations consisting of normal subjects and autoi mmune disease patients (P < 0.01). The immunodominant epitopes were at the amino termini of both antigens and absorption studies showed that the major ity of autoantibodies were not cross-reactive. Autoantibodies to p62 and Ko c were present in approximately similar frequencies in a variety of maligna ncies and the immune responses appeared to be independent of each other. Th e immune responses might be related to overexpression or dysregulation of p 62 and Koc in some tumors. (C) 2001 Academic Press.