Benzyladenine-preconditioning in germinating mungbean seedlings stimulatesaxillary buds in cotyledonary nodes resulting in multiple shoot regeneration

Citation
Ra. Avenido et K. Hattori, Benzyladenine-preconditioning in germinating mungbean seedlings stimulatesaxillary buds in cotyledonary nodes resulting in multiple shoot regeneration, BREED SCI, 51(2), 2001, pp. 137-142
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
BREEDING SCIENCE
ISSN journal
0536-3683 → ACNP
Volume
51
Issue
2
Year of publication
2001
Pages
137 - 142
Database
ISI
SICI code
0536-3683(200106)51:2<137:BIGMSS>2.0.ZU;2-3
Abstract
Tissue culture and histological studies were done to ascertain the effects of benzyladenine (BA)-preconditioning on direct shoot regeneration from cot yledonary node explants of mungbean. The highest direct shoot regeneration (93.3 %), double-sided shoot initiation (65.0 %) and average number of shoo ts per explant (2.7 shoots) were obtained when Pag-asa 7 explants are excis ed from seedlings germinated in basal medium (BM) consisting of MS salts an d B5 vitamins with 1.0 mg l(-1) BA, and subsequently cultured in fresh medi um of the same composition. Histological inspection of sections from germin ating seeds in BM and BM + 1.0 mg l(-1) BA sampled at 1 to 4 days from germ ination revealed bigger and developmentally more advanced axillary shoots i n BA-preconditioned seedlings, a built-in advantage which could attest to t he improved regenerability in BA-treated seedling explants over the control (BM only). During shoot initiation from explants, two treatments namely do ubling of the concentration BA to 2.0 mg l(-1) and the addition of a non-io nic surfactant, Pluronic F-68 did not further increase the responses, but g ave results comparable with those obtained using BM + 1.0 mg l(-1) BA alone . Conversely, a BA-thidiazuron (TDZ) combination (each at 1.0 mg l(-1)) whe n added to BM significantly reduced shoot induction to only 48.3 % possibly due to increased callusing at the nodes in 55 % of the explants. The impor tance and applicability of the regeneration system in tissue culture and/or genetic manipulations of mungbean and other Asiatic Vigna species is discu ssed.