The methionine biosynthetic pathway from homoserine in Pseudomonas putida involves the metW, metX, metZ, metH and metE gene products

Alaminos, M Ramos, JL

M. Alaminos et Jl. Ramos, The methionine biosynthetic pathway from homoserine in Pseudomonas putida involves the metW, metX, metZ, metH and metE gene products, ARCH MICROB, 176(1-2), 2001, pp. 151-154

28

INGLESE

Article

Microbiology

ARCHIVES OF MICROBIOLOGY

0302-8933 → ACNP

176

1-2

2001

151 - 154

ISI

0302-8933(200107)176:1-2<151:TMBPFH>2.0.ZU;2-5

Biosynthesis of methionine from homoserine in Pseudomonas putida takes plac e in three steps. The first step is the acylation of homoserine to yield an acyl-L-homoserine. This reaction is catalyzed by the products of the metXW genes and is equivalent to the first step in enterobacteria, gram-positive bacteria and fungi, except that in these microorganisms the reaction is ca talyzed by a single polypeptide (the product of the metA gene in Escherichi a coli and the met5 gene product in Neurospora crassa). In Pseudomonas puti da, as in gram-positive bacteria and certain fungi, the second and third st eps are a direct sulfhydrylation that converts the O-acyl-L-homoserine into homocysteine and further methylation to yield methionine. The latter react ion can be mediated by either of the two methionine synthetases present in the cells.