The methionine biosynthetic pathway from homoserine in Pseudomonas putida involves the metW, metX, metZ, metH and metE gene products

Citation
M. Alaminos et Jl. Ramos, The methionine biosynthetic pathway from homoserine in Pseudomonas putida involves the metW, metX, metZ, metH and metE gene products, ARCH MICROB, 176(1-2), 2001, pp. 151-154
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
176
Issue
1-2
Year of publication
2001
Pages
151 - 154
Database
ISI
SICI code
0302-8933(200107)176:1-2<151:TMBPFH>2.0.ZU;2-5
Abstract
Biosynthesis of methionine from homoserine in Pseudomonas putida takes plac e in three steps. The first step is the acylation of homoserine to yield an acyl-L-homoserine. This reaction is catalyzed by the products of the metXW genes and is equivalent to the first step in enterobacteria, gram-positive bacteria and fungi, except that in these microorganisms the reaction is ca talyzed by a single polypeptide (the product of the metA gene in Escherichi a coli and the met5 gene product in Neurospora crassa). In Pseudomonas puti da, as in gram-positive bacteria and certain fungi, the second and third st eps are a direct sulfhydrylation that converts the O-acyl-L-homoserine into homocysteine and further methylation to yield methionine. The latter react ion can be mediated by either of the two methionine synthetases present in the cells.