Induction of butane consumption in Pseudomonas butanovora

Sayavedra-Soto, LA Byrd, CM Arp, DJ

La. Sayavedra-soto et al., Induction of butane consumption in Pseudomonas butanovora, ARCH MICROB, 176(1-2), 2001, pp. 114-120

40

INGLESE

Article

Microbiology

ARCHIVES OF MICROBIOLOGY

0302-8933 → ACNP

176

1-2

2001

114 - 120

ISI

0302-8933(200107)176:1-2<114:IOBCIP>2.0.ZU;2-V

The induction of the enzyme activities involved in butane metabolism in Pse udomonas butanovora was characterized. P. butanovora was grown on butane or its metabolites, both singly and in mixtures with other growth substrates. Celts grown in each of the butane metabolites readily consumed the growth substrate and downstream metabolites, but consumed the upstream butane meta bolites more slowly. Upstream activities in the butane metabolism could be induced by downstream metabolites, but to much lower levels than with the p rimary substrate. The induction of butane oxidation was not repressed when P. butanovora was grown or incubated in a mixture of butane and 1-butanol, butyraldehyde or butyrate. However, no induction of butane consumption was observed in a mixture of butane and lactate, which is indicative of catabol ite repression. In lactate-grown cells that were rid of the growth substrat e and incubated with butane and acetylene (to inactivate newly formed butan e monooxygenase), the consumption of butane, 1-butanol and butyraldehyde co nsumption was not induced. The overall results suggest an independent regul atory mechanism for each of the enzyme activities in butane metabolism. In addition, a low, constitutive butane oxidation was observed in cells grown on substrates other than butane metabolites.