Ethanedisulfonate is degraded via sulfoacetaldehyde in Ralstonia sp strainEDS1

Citation
K. Denger et Am. Cook, Ethanedisulfonate is degraded via sulfoacetaldehyde in Ralstonia sp strainEDS1, ARCH MICROB, 176(1-2), 2001, pp. 89-95
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
176
Issue
1-2
Year of publication
2001
Pages
89 - 95
Database
ISI
SICI code
0302-8933(200107)176:1-2<89:EIDVSI>2.0.ZU;2-K
Abstract
Aerobic enrichment cultures (11) yielded three cultures able to utilise eth ane-1,2-disulfonate as sole source of carbon and energy in salts medium. Tw o pure cultures were obtained and we worked with strain EDS1, which was ass igned to the genus Ralstonia on the basis of its 16S rDNA sequence and simp le taxonomic tests. Strain EDS1 utilised at least seven alkane(di)sulfonate s, ethane-1,2-disulfonate, taurine, isethionate, sulfoacetate, sulfoacetald ehyde and propane-1,3-disulfonate, as well as methanesulfonate and formate. Growth with ethanedisulfonate was concomitant with substrate disappearance and the formation of 2 mol sulfate per mol substrate. The growth yield, 7 g protein (mol C)(-1), indicated quantitative utilisation of the substrate. Ethanedisulfonate-dependent oxygen uptake of whole cells during growth ros e to a maximum before the end of growth and then sank rapidly; this was int erpreted as evidence for an inducible desulfonative oxygenase that was not active in cell extracts. Inducible sulfoacetaldehyde sulfo-lyase was detect ed at high activity. Inducible degradation of taurine or isethionate or sul foacetate via sulfoacetaldehyde sulfo-lyase is interpreted from the data.