Aerobic enrichment cultures (11) yielded three cultures able to utilise eth
ane-1,2-disulfonate as sole source of carbon and energy in salts medium. Tw
o pure cultures were obtained and we worked with strain EDS1, which was ass
igned to the genus Ralstonia on the basis of its 16S rDNA sequence and simp
le taxonomic tests. Strain EDS1 utilised at least seven alkane(di)sulfonate
s, ethane-1,2-disulfonate, taurine, isethionate, sulfoacetate, sulfoacetald
ehyde and propane-1,3-disulfonate, as well as methanesulfonate and formate.
Growth with ethanedisulfonate was concomitant with substrate disappearance
and the formation of 2 mol sulfate per mol substrate. The growth yield, 7
g protein (mol C)(-1), indicated quantitative utilisation of the substrate.
Ethanedisulfonate-dependent oxygen uptake of whole cells during growth ros
e to a maximum before the end of growth and then sank rapidly; this was int
erpreted as evidence for an inducible desulfonative oxygenase that was not
active in cell extracts. Inducible sulfoacetaldehyde sulfo-lyase was detect
ed at high activity. Inducible degradation of taurine or isethionate or sul
foacetate via sulfoacetaldehyde sulfo-lyase is interpreted from the data.