Polo-like kinase is required for the fragmentation of pericentriolar Golgistacks during mitosis

Citation
C. Sutterlin et al., Polo-like kinase is required for the fragmentation of pericentriolar Golgistacks during mitosis, P NAS US, 98(16), 2001, pp. 9128-9132
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
98
Issue
16
Year of publication
2001
Pages
9128 - 9132
Database
ISI
SICI code
0027-8424(20010731)98:16<9128:PKIRFT>2.0.ZU;2-W
Abstract
The pericentriolar stacks of Golgi cisternae undergo extensive reorganizati on during mitosis in mammalian cells. GM130 and GRASP65 (Golgi reassembly s tacking protein of 65 kDa) are Golgi-associated proteins that are targets o f mitotic kinases, and they have also been implicated in the reorganization of the Golgi structure during cell division. Previous studies have reporte d that mitogen-activated protein kinase kinase-1 (MEK1} and Cdc2 protein ki nases are involved in these dynamic changes in the Golgi structure. More re cently, the mitotic polo-like kinase (Plk} has been shown to interact with and phosphorylate GRASP65. Here, we provide evidence that Plk is involved i n the mitosis-specific fragmentation of the Golgi apparatus. The addition o f kinase-defective Plk or immunodepletion of Plk disrupts the fragmentation process. Furthermore, Golgi fragmentation is inhibited by the addition of either full-length or truncated GRASP65. These findings suggest that phosph arylation of GRASP65 by Plk may be a critical event in the reorganization o f the Golgi structure during mitosis.