Rat parathyroid allotransplantation: Influence of MHC antigen expression on graft survival

Citation
S. Timm et al., Rat parathyroid allotransplantation: Influence of MHC antigen expression on graft survival, MICROSURG, 21(5), 2001, pp. 221-222
Citations number
4
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
MICROSURGERY
ISSN journal
0738-1085 → ACNP
Volume
21
Issue
5
Year of publication
2001
Pages
221 - 222
Database
ISI
SICI code
0738-1085(2001)21:5<221:RPAIOM>2.0.ZU;2-#
Abstract
MHC antigen expression in parathyroid tissue and its influence on graft sur vival after allogeneic transplantation were investigated using a heterotopi c rat transplantation model. MHC class I and II expression in parathyroid t issue of Lewis (LEW), Dark Agouti (DA), and Wistar-Furth (WF) rats was anal ysed semi-quantitatively by using immunohistochemistry. MHC class I express ion was strong in DA, moderate in WF, and weak in LEW rats parenchyma, wher eas MHC class II expression was negative. In the interstitium of all invest igated tissue specimens, the proportion of MHC class II-expressing cells wa s low. Additionally, four groups were transplanted: 1) LEW to LEW, 2) DA to DA, 3) LEW to DA, and 4) WF to LEW. After syngeneic transplantation, graft survival could be documented over the whole observation period. A median g raft survival of 20 (+/-2) days was observed after transplantation from LEW to DA, whereas grafts in the group WF to LEW were rejected after 13 (+/-1) days. The number of intra-graft leucocytes expressing MHC class II molecul es was the same in all groups, whereas increased levels of MHC class I in p arathyroid tissue before transplantation resulted in a more rapid rejection . These results indicate that immunogenicity of rat parathyroid tissue migh t be determined by the amount of MHC class I expressed in donor parenchymal cells. Further experiments are necessary to validate this observation. (C) 2001 Wiley-Liss, Inc.