Clinical isolates of non-0157 shiga toxin-producing Escherichia coli: Serotypes, virulence characteristics, and molecular profiles of strains of the same serotype

Citation
M. Eklund et al., Clinical isolates of non-0157 shiga toxin-producing Escherichia coli: Serotypes, virulence characteristics, and molecular profiles of strains of the same serotype, J CLIN MICR, 39(8), 2001, pp. 2829-2834
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
39
Issue
8
Year of publication
2001
Pages
2829 - 2834
Database
ISI
SICI code
0095-1137(200108)39:8<2829:CIONST>2.0.ZU;2-1
Abstract
All human Shiga toxin-producing Escherichia coli (STEC) non-0157 strains (n = 56) isolated in Finland from 1990 to August 2000 were characterized for the O:H serotype, stx(1) and stx(2) genes, production of enterohemolysin, a nd sensitivity to 12 antimicrobial agents. Strains of the same serotype wer e genotyped by pulsed-field gel electrophoresis (PFGE) after XbaI restricti on of total DNA. The 56 non-0157 isolates belonged to 29 serotypes. Two of the serotypes (O102:H7 and OX181:H49) have not previously been described as being associated with STEC infections in humans or isolated from animals. Thirty-four strains (61%) within seven serotypes (O103:H2 [14 isolates], O2 6:H11 [6 isolates], O145:H28 [4 isolates], O145:HNM [3 isolates], O15:HNM [ 3 isolates], OX174:H21 [2 isolates], and O Rough:HNM [2 isolates]) were rep resented by more than one isolate. Of these strains, O103:H2 isolates were divided into seven, O26:H11 isolates were divided into four, and the rest w ithin a serotype were divided into two genotypes in PFGE. In PCR, 31 (55%) of the 56 strains were positive for the stx(2) gene only and 24 strains (43 %) were positive for stx(1) only. One strain (O43:H2) carried both stx(1) a nd stx(2). Forty-two strains (75%) produced enterohemolysin, and 39 strains (70%) possessed the eae gene. Of the latter 39 strains, 36 (92%) were ente rohemolytic, whereas only 6 (35%) of the 17 isolates lacking the eae gene w ere enterohemolytic (P < 0.001). The majority of the strains (44 strains, 7 9%) were sensitive to all 12 antimicrobials tested. Of the 56 strains, 20 ( 36%) were associated with small family outbreaks in nine families and 14 (2 5%) were associated with recent travel abroad.