Metabolic activity in disrupted human anterior cruciate ligament. Evaluation of procollagen mRNA expression in 29 patients

Citation
N. Fukui et al., Metabolic activity in disrupted human anterior cruciate ligament. Evaluation of procollagen mRNA expression in 29 patients, JOINT BONE, 68(4), 2001, pp. 318-326
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Rheumatology
Journal title
JOINT BONE SPINE
ISSN journal
1297-319X → ACNP
Volume
68
Issue
4
Year of publication
2001
Pages
318 - 326
Database
ISI
SICI code
1297-319X(2001)68:4<318:MAIDHA>2.0.ZU;2-C
Abstract
Background. Although the anterior cruciate ligament heals poorly, recent re ports demonstrated that this ligament has a rather active metabolic activit y after disruption. The details of such activity are, however, not well kno wn. Objective. To determine the localization of metabolic activity and its influential factors in the human disrupted anterior cruciate ligament. Pati ents and methods. The tibial remnants of completely disrupted anterior cruc iate ligaments were obtained en bloc from 29 patients 2 to 94 days after in jury. The expression of pro alpha1(I) and pro alpha1 (III) collagen mRNA in the ligament substance was evaluated by non-radioactive in situ hybridizat ion. The relationship was studied between the mRNA expression and the patie nt's age, time after injury, presence of associated injury, or the conditio n of the ligament stump, respectively. Results. The expression of procollag en mRNA was observed in 72% of the cases, most often at the torn end but le ss frequently around the tibial insertion. The expression of type IH procol lagen mRNA was always observed within the area of type I expression. While the other factors had no significant relation with the expression, the exte nt of synovium coverage over the ligament stumps had a positive correlation with procollagen mRNA expression at the torn ends. Conclusion. The metabol ic activity in a disrupted human anterior cruciate ligament shows considera ble disparity among the cases in its localization. The pattern of ligament disruption could have a significant correlation with such activity at the t orn ends. (C) 2001 editions scientifiques et medicales Elsevier SAS.