Microfabricated disposable DNA sensors based on enzymatic amplification electrochemical detection

Citation
Dk. Xu et al., Microfabricated disposable DNA sensors based on enzymatic amplification electrochemical detection, ELECTROANAL, 13(10), 2001, pp. 882-887
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ELECTROANALYSIS
ISSN journal
1040-0397 → ACNP
Volume
13
Issue
10
Year of publication
2001
Pages
882 - 887
Database
ISI
SICI code
1040-0397(200107)13:10<882:MDDSBO>2.0.ZU;2-5
Abstract
A new electrochemical biosensing method was developed based on the immobili zation of DNA probes on photolithographic gold film electrodes by self-asse mbled monolayer technique. The complementary sequence was hybridized to the modified electrode and the hybridization reaction was carried out at 37 de greesC for 1 h. Avidin-akaline phosphatase was coupled to the hybrid throug h avidin-biotin complex and hydrolyzed to produce an electroactive alpha -n aphthol, which could be detected by different pulse voltammetry (DPV). Nume rous factors including the concentration of the substrate and incubation ti me are optimized to maximize the sensitivity and speed in the assay time. T he sensors were employed to detect PCR products relative to the hepatitis B plasmid. The method has a linear calibration range of 1.5 nmol/L-50 nmol/L and the detection limit is 30 fmol.