Assignment of T cell receptor (TCR) alpha-chain gene (A), beta-chain gene (B), gamma-chain gene (G), and delta-chain gene (D) loci on swine chromosomes by in situ hybridization and radiation hybrid mapping

Citation
H. Hiraiwa et al., Assignment of T cell receptor (TCR) alpha-chain gene (A), beta-chain gene (B), gamma-chain gene (G), and delta-chain gene (D) loci on swine chromosomes by in situ hybridization and radiation hybrid mapping, CYTOG C GEN, 93(1-2), 2001, pp. 94-99
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CYTOGENETICS AND CELL GENETICS
ISSN journal
0301-0171 → ACNP
Volume
93
Issue
1-2
Year of publication
2001
Pages
94 - 99
Database
ISI
SICI code
0301-0171(2001)93:1-2<94:AOTCR(>2.0.ZU;2-X
Abstract
Using the cDNA sequence of porcine T-cell receptor (TCR) alpha-, beta-, gam ma-, and delta -chain genes, we screened a porcine BAC library to isolate c lones containing these genes. The isolated BAC clones were confirmed to car ry these TCR genes by partial nucleotide sequencing. Among the clones obtai ned in the present screening, two clones carried both the TCR alpha -chain gene (TRA) and the TCR delta -chain gene (TRD) while one clone each carried only the sequence of either TRA or TRD. This observation demonstrated that TRA and TRD are localized in close proximity on a swine chromosome. Also t wo clones contained the sequence of the TCR beta -chain gene (TRB), and two clones contained the sequence of TCR gamma -chain gene (TRG). Fluorescence in situ hybridization using the above BAC clones as probes revealed that T RA and TRD, TRB, and TRG loci reside on swine chromosomes 7q15.3 --> q21, 1 8q11.3 --> q12, and 9q21 ---> q22, respectively. The chromosome positions o f TRA and TRB are consistent with those determined by somatic cell hybrid a nalysis (Rettenberger et al., 1996). In addition, RH mapping of these genes was performed using the INRA-University of Minnesota RH panel DNA. The res ult confirmed the position of TRA and TRB reported earlier (http://imprh.to ulouse.inra.fr/), and further demonstrated that TRG was located 11 cR away from genetic marker SW989 toward the marker S0019. Copyright (C) 2001 S. Ka rger AG, Basel.