Assignment of T cell receptor (TCR) alpha-chain gene (A), beta-chain gene (B), gamma-chain gene (G), and delta-chain gene (D) loci on swine chromosomes by in situ hybridization and radiation hybrid mapping
H. Hiraiwa et al., Assignment of T cell receptor (TCR) alpha-chain gene (A), beta-chain gene (B), gamma-chain gene (G), and delta-chain gene (D) loci on swine chromosomes by in situ hybridization and radiation hybrid mapping, CYTOG C GEN, 93(1-2), 2001, pp. 94-99
Using the cDNA sequence of porcine T-cell receptor (TCR) alpha-, beta-, gam
ma-, and delta -chain genes, we screened a porcine BAC library to isolate c
lones containing these genes. The isolated BAC clones were confirmed to car
ry these TCR genes by partial nucleotide sequencing. Among the clones obtai
ned in the present screening, two clones carried both the TCR alpha -chain
gene (TRA) and the TCR delta -chain gene (TRD) while one clone each carried
only the sequence of either TRA or TRD. This observation demonstrated that
TRA and TRD are localized in close proximity on a swine chromosome. Also t
wo clones contained the sequence of the TCR beta -chain gene (TRB), and two
clones contained the sequence of TCR gamma -chain gene (TRG). Fluorescence
in situ hybridization using the above BAC clones as probes revealed that T
RA and TRD, TRB, and TRG loci reside on swine chromosomes 7q15.3 --> q21, 1
8q11.3 --> q12, and 9q21 ---> q22, respectively. The chromosome positions o
f TRA and TRB are consistent with those determined by somatic cell hybrid a
nalysis (Rettenberger et al., 1996). In addition, RH mapping of these genes
was performed using the INRA-University of Minnesota RH panel DNA. The res
ult confirmed the position of TRA and TRB reported earlier (http://imprh.to
ulouse.inra.fr/), and further demonstrated that TRG was located 11 cR away
from genetic marker SW989 toward the marker S0019. Copyright (C) 2001 S. Ka
rger AG, Basel.