Cytokine production and helper T cell subsets in Vogt-Koyanagi-Harada's disease

Citation
Ym. Imai et al., Cytokine production and helper T cell subsets in Vogt-Koyanagi-Harada's disease, CURR EYE R, 22(4), 2001, pp. 312-318
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
CURRENT EYE RESEARCH
ISSN journal
0271-3683 → ACNP
Volume
22
Issue
4
Year of publication
2001
Pages
312 - 318
Database
ISI
SICI code
0271-3683(2001)22:4<312:CPAHTC>2.0.ZU;2-S
Abstract
Purpose. To investigate helper T (Th) cell subsets and cytokine production in patients with Vogt-Koyanagi-Harada's (VKH) disease. Methods. Nine patients in the acute stage of VKH disease and 9 healthy cont rols were studied. Interferon-gamma (IFN-gamma), interleukin-2 (IL-2) and I L-4 mRNA from peripheral blood mononuclear cells (PBMC) was detected by rev erse transcriptase-polymerase chain reaction. Cytokine levels in plasma, ce rebrospinal fluid (CSF) and PBMC culture supernatant were measured by enzym e-linked immunosorbent assay (ELISA). The proportions of each cytokine-prod ucing CD4+ or CD8+ cells in PBMC cultured with or without anti-CD3 antibody stimulation were analyzed by flow cytometry. Results. We observed a significant increase of cytokine mRNA positive cases of VKH patients in comparison with controls only in IFN-gamma mRNA detecti on from PBMC. ELISA detected IFN-gamma in CSF from only 1 patient and no cy tokine in plasma. The levels of IFN-gamma and IL-2 were significantly highe r in the stimulated cell culture supernatant of the patients than those of controls. The proportions of IFN-gamma- or IL-2-producing CD4+ cells were s ignificantly higher in the patients than in controls in both stimulated and unstimulated conditions. However, no significant difference was found in I L-4-producing CD4+ cells. In CD8+ cells, significant difference was found o nly in IL-2-producing cells in the stimulated condition. Conclusions. Th cells from VKH patients produced predominantly Th1 cytokine s, especially after stimulation in vitro. It is therefore suggested that ac tivated Th cells produce predominantly Th1 cytokines, which then produce pa thologic changes.