Polymorphisms of chemokine receptors and its ligand alleles influencing genetic susceptibity to HIV-1 infection in eight ethnic groups in Chinese mainland

Citation
Fs. Wang et al., Polymorphisms of chemokine receptors and its ligand alleles influencing genetic susceptibity to HIV-1 infection in eight ethnic groups in Chinese mainland, CHIN SCI B, 46(12), 2001, pp. 1019-1023
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
CHINESE SCIENCE BULLETIN
ISSN journal
1001-6538 → ACNP
Volume
46
Issue
12
Year of publication
2001
Pages
1019 - 1023
Database
ISI
SICI code
1001-6538(200106)46:12<1019:POCRAI>2.0.ZU;2-Z
Abstract
polymorphisms of HIV-1 resistant genes in indigenous Chinese populations. T he aim of this study is to identify the allelic frequencies of the chemokin e and chemokine receptor genes in the Chinese mainland. Genomic DNA samples extracted from whole blood of 2318 subjects were analyzed by using PCR or PCR/restriction fragment length polymorphism (RFLP) assays, and further con firmed by direct DNA sequencing. Higher frequencies of mutant CCR2-641 (19. 15%-28.79%) and SDFI-3'A (19.10%-29.86%) alleles were found in subjects of 8 ethnic groups in the Chinese mainland. In contrast, the Delta 32 mutation in CCR5 gene occurs at a very low frequency (0.0016, n=1287) in Han popula tion. A relatively high frequency of CCR5-wt/Delta 32 heterozygotes was obs erved in Uygurian and Mongolian populations. No Delta 32 mutation allele wa s detected in Tibetan and other 4 ethnic groups in Yunnan Province. There w as no CCR5-m303 mutation in subjects of any ethnic group in the Chinese mai nland. Our results suggest that the CCR5-Delta 32 mutation is not a major r esistant factor against HIV-1 infection and disease progression in Han, Tib etan and other ethnic groups in Yunnan Province. Whether higher frequencies of CCR2-641 and SDF1-3'A alleles constitute major genetic resistant factor s or not remains to be clarified.