Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313

Citation
S. Palit et R. Banerjee, Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313, BRAZ ARCH B, 44(1), 2001, pp. 107-111
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
ISSN journal
1516-8913 → ACNP
Volume
44
Issue
1
Year of publication
2001
Pages
107 - 111
Database
ISI
SICI code
1516-8913(200103)44:1<107:OOEPFR>2.0.ZU;2-4
Abstract
The extraction of alpha- amylase in the solid state fermentation of wheat b ran by Bacillus circulans GRS313 was optimized. Among various solvents test ed, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30 degrees C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity o f the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the Is t and 2nd washes respectively and only 3684 U/g was recovered in the 3rd wa sh.