The expression of a barley HvNAS1 nicotianamine synthase gene promoter-gusfusion gene in transgenic tobacco is induced by Fe-deficiency in roots

Authors
Higuchi, K Tani, M Nakanishi, H Yoshiwara, T Goto, F Nishizawa, NK Mori, S
Citation
K. Higuchi et al., The expression of a barley HvNAS1 nicotianamine synthase gene promoter-gusfusion gene in transgenic tobacco is induced by Fe-deficiency in roots, BIOS BIOT B, 65(7), 2001, pp. 1692-1696
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
0916-8451 → ACNP
Volume
65
Issue
7
Year of publication
2001
Pages
1692 - 1696
Database
ISI
SICI code
0916-8451(200107)65:7<1692:TEOABH>2.0.ZU;2-W
Abstract
Nicotianamine (NA) is a precursor for mugineic acid-family phytosiderophore s, which are a critical component of the Fe aquisition process in graminace ous plants. In addition, nicotianamine synthase (NAS) is strongly induced i n these plants by Fe deficiency. NA is essential for Fe metabolism also in dicots, but NAS is not induced by Fe deficiency. We introduced a barley HvN AS1 promoter-gus fusion gene into tobacco. GUS activity was induced in the roots of these plants by Fe deficiency, and was constitutively expressed at a low level in their leaves.