Levoglucosan kinase involved in citric acid fermentation by Aspergillus niger CBX-209 using levoglucosan as sole carbon and energy source

Citation
Xl. Zhuang et al., Levoglucosan kinase involved in citric acid fermentation by Aspergillus niger CBX-209 using levoglucosan as sole carbon and energy source, BIO BIOENER, 21(1), 2001, pp. 53-60
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOMASS & BIOENERGY
ISSN journal
0961-9534 → ACNP
Volume
21
Issue
1
Year of publication
2001
Pages
53 - 60
Database
ISI
SICI code
0961-9534(2001)21:1<53:LKIICA>2.0.ZU;2-R
Abstract
Conditions were optimized for the production of citric acid by a mutant A. niger CBX-209 using levoglucosan derived from pyrolysis of wastepaper as a sole carbon and energy source in a simple medium. The optimum concentration of levoglucosan and wheat bran in the medium was 8% and 3%, respectively, at an optimum initial pH between 5.5 and 6.0 at 35 degreesC. During ferment ation, direct formation of glucose 6-phosphate from levoglucosan in the pre sence of ATP and Mg2+ as observed by HPLC in the reaction with both cell ex tracts and partially purified enzyme, suggested that the enzyme acting on l evoglucosan is a kinase. Time-course changes in the levels of this special levoglucosan kinase in A. niger CBX-209 grown on levoglucosan and glucose r evealed that levoglucosan kinase was an inductive enzyme. (C) 2001 Elsevier Science Ltd. All rights reserved.