Interfering effect of diphtheria-tetanus-acellular pertussis combined (DTaP) vaccines on the bacterial endotoxin test

Citation
M. Ochiai et al., Interfering effect of diphtheria-tetanus-acellular pertussis combined (DTaP) vaccines on the bacterial endotoxin test, BIOLOGICALS, 29(1), 2001, pp. 55-58
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
BIOLOGICALS
ISSN journal
1045-1056 → ACNP
Volume
29
Issue
1
Year of publication
2001
Pages
55 - 58
Database
ISI
SICI code
1045-1056(200103)29:1<55:IEODPC>2.0.ZU;2-W
Abstract
We evaluated applicability of the endotoxin test to DTaP vaccines. Although no DTaP vaccine batches showed immediate interference with the activating activity of spiked endotoxin on the clotting of Limulus amaebocyte lysate ( LAL), a gradually progressing interference depending on the time after spik ing was seen for some of the batches. The interfering DTaP vaccine batches, however, showed no significant effect on in vitro TNF-alpha induction in R AW264.7 cells and pyrogenicity in rabbits of spiked endotoxin. Aluminium hy droxide gel in the vaccines was suggested to be one of the causes of the in terference. Accordingly, a careful evaluation of the interfering effect was assumed crucial for the effective application of the endotoxin test to che ck residual biological activity of endotoxin in DTaP vaccines.