Mapping proteins of the 50S subunit from Escherichia coli ribosomes

Citation
R. Willumeit et al., Mapping proteins of the 50S subunit from Escherichia coli ribosomes, BBA-GENE ST, 1520(1), 2001, pp. 7-20
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
0167-4781 → ACNP
Volume
1520
Issue
1
Year of publication
2001
Pages
7 - 20
Database
ISI
SICI code
0167-4781(20010730)1520:1<7:MPOT5S>2.0.ZU;2-D
Abstract
Mapping of protein positions in the ribosomal subunits was first achieved f or the 30S subunit by means of neutron scattering about 15 years ago. Since the 50S subunit is almost twice as large as the 30S subunit and consists o f more proteins, it was difficult to apply classical contrast variation tec hniques for the localisation of the proteins. Polarisation dependent neutro n scattering (spin-contrast variation) helped to overcome this restriction. Here a map of 14 proteins within the 50S subunit from Escherichia coli rib osomes is presented including the proteins L17 and L20 that are not present in archeal ribosomes. The results are compared with the recent crystallogr aphic map of the 50S subunit from the archea Haloarcula marismortui. (C) 20 01 Elsevier Science BY. All rights reserved.