Involvement of a novel Arabidopsis phospholipase D, AtPLD delta, in dehydration-inducible accumulation of phosphatidic acid in stress signalling

Katagiri, T Takahashi, S Shinozaki, K

T. Katagiri et al., Involvement of a novel Arabidopsis phospholipase D, AtPLD delta, in dehydration-inducible accumulation of phosphatidic acid in stress signalling, PLANT J, 26(6), 2001, pp. 595-605

35

INGLESE

Article

Plant Sciences","Animal & Plant Sciences

PLANT JOURNAL

0960-7412 → ACNP

26

6

2001

595 - 605

ISI

0960-7412(200106)26:6<595:IOANAP>2.0.ZU;2-3

Phospholipid metabolism is involved in plant responses to drought and salin ity stress. To investigate the role of phospholipase D (PLD) and its produc t phosphatidic acid (PtdOH) in stress signalling, we isolated a novel PLD c DNA, designated AtPLD delta, by screening a cDNA library prepared from dehy drated Arabidopsis thaliana. The AtPLD delta protein, of 868 amino acids, h as a putative catalytic domain and a C2 domain that is involved in Ca2+/pho spholipid binding. The AtPLD delta mRNA accumulated in response to dehydrat ion and high salt stress. Histochemical analysis showed that the AtPLD delt a gene is strongly expressed in the vascular tissues of cotyledons and leav es under dehydration stress conditions. Under normal growth conditions, AtP LD delta was expressed in roots, leaves, stems and flowers but not in siliq ues. We showed that dehydration stimulates the accumulation of PtdOH. The a ccumulation of PtdOH in response to dehydration was significantly suppresse d in AtPLD delta antisense transgenic plants. These results suggest that At PLD delta may be involved in PtdOH accumulation in the dehydration stress r esponse.