Involvement of a novel Arabidopsis phospholipase D, AtPLD delta, in dehydration-inducible accumulation of phosphatidic acid in stress signalling

Citation
T. Katagiri et al., Involvement of a novel Arabidopsis phospholipase D, AtPLD delta, in dehydration-inducible accumulation of phosphatidic acid in stress signalling, PLANT J, 26(6), 2001, pp. 595-605
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
26
Issue
6
Year of publication
2001
Pages
595 - 605
Database
ISI
SICI code
0960-7412(200106)26:6<595:IOANAP>2.0.ZU;2-3
Abstract
Phospholipid metabolism is involved in plant responses to drought and salin ity stress. To investigate the role of phospholipase D (PLD) and its produc t phosphatidic acid (PtdOH) in stress signalling, we isolated a novel PLD c DNA, designated AtPLD delta, by screening a cDNA library prepared from dehy drated Arabidopsis thaliana. The AtPLD delta protein, of 868 amino acids, h as a putative catalytic domain and a C2 domain that is involved in Ca2+/pho spholipid binding. The AtPLD delta mRNA accumulated in response to dehydrat ion and high salt stress. Histochemical analysis showed that the AtPLD delt a gene is strongly expressed in the vascular tissues of cotyledons and leav es under dehydration stress conditions. Under normal growth conditions, AtP LD delta was expressed in roots, leaves, stems and flowers but not in siliq ues. We showed that dehydration stimulates the accumulation of PtdOH. The a ccumulation of PtdOH in response to dehydration was significantly suppresse d in AtPLD delta antisense transgenic plants. These results suggest that At PLD delta may be involved in PtdOH accumulation in the dehydration stress r esponse.