Identification and subtyping of Trichophyton mentagrophytes by random amplified polymorphic DNA

Citation
Ja. Kim et al., Identification and subtyping of Trichophyton mentagrophytes by random amplified polymorphic DNA, MYCOSES, 44(5), 2001, pp. 157-165
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
MYCOSES
ISSN journal
0933-7407 → ACNP
Volume
44
Issue
5
Year of publication
2001
Pages
157 - 165
Database
ISI
SICI code
0933-7407(2001)44:5<157:IASOTM>2.0.ZU;2-Z
Abstract
Trichophyton mentagrophytes is one of the most common pathogens for human a nd animal dermatophytoses and known as a complex species with variable morp hology, and ecologic and genetic backgrounds. We performed random amplified polymorphic DNA (RAPD) analysis on 43 human and 18 animal isolates of T. m entagrophytes along with other 10 anamorphic species of dermatophytes and t hree teleomorphic species of 7, mentagrophtes, Using RAPD analysis with pri mer 5'-ATGGATC(G,C)(G,C)C-3' (ATGS), all T. mentagrophytes strains produced identical band patterns with those of; Arthroderma vanbreuseghemii, one of the teleomorphs of T. mentagrophytes, regardless of their phenotypes. Ther efore, 7 metagrophytes could be identified by RAPD analysis with primer ATG S Using RAPD analysis with primer 5'-ATGGATCGCC-3' (ATG) on T. mentagrophyt es, human isolates yielded two distinct subgroups related by their colony m orphologies at the time of primary isolation from patients. Three morpholog ic types - cottony, powdery and persicolor - revealed identical bands where as the granular type lacked one minor band (0.74 kbp), Animal isolates of 7 . mentagrophytes produced five band patterns and some of them were identica l with those of human isolates. With primer 5'GAAGGCTCCC-3' (OPAO-15), anim al isolates of T. mentagrophytes showed diverse band patterns in contrast t o the uniform band pattern of human isolates. These results suggest that RA PD analysis may be a useful tool to identify and subtype 7. mentagrophytes complex.