Mediator, not holoenzyme, is directly recruited to the heat shock promoterby HSF upon heat shock

Citation
Jm. Park et al., Mediator, not holoenzyme, is directly recruited to the heat shock promoterby HSF upon heat shock, MOL CELL, 8(1), 2001, pp. 9-19
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR CELL
ISSN journal
1097-2765 → ACNP
Volume
8
Issue
1
Year of publication
2001
Pages
9 - 19
Database
ISI
SICI code
1097-2765(200107)8:1<9:MNHIDR>2.0.ZU;2-V
Abstract
Activators of RNA polymerase II (Pol II) transcription have been shown to b ind several coactivators and basal factors in vitro. Whether such interacti ons play a primary regulatory role in recruiting these factors to activator -associated chromosomal target sites in living cells remains unclear. Here, we show that upon heat shock the Pol II-free form of Mediator is rapidly r ecruited to HSF binding sites. Unlike the TAFs and Pot II, the interaction between Mediator and HSF on chromosomal loci is direct and mechanistically separable from the preinitiation complex assembly step. Therefore, the acti vator-Mediator interaction likely underlies the initiation of signal transf er from enhancer-bound activators to the basal transcription machinery.