Radiation hybrid mapping of 11 alpha and beta nicotinic acetylcholine receptor genes in Rattus norvegicus

Citation
J. Tseng et al., Radiation hybrid mapping of 11 alpha and beta nicotinic acetylcholine receptor genes in Rattus norvegicus, MOL BRAIN R, 91(1-2), 2001, pp. 169-173
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
MOLECULAR BRAIN RESEARCH
ISSN journal
0169-328X → ACNP
Volume
91
Issue
1-2
Year of publication
2001
Pages
169 - 173
Database
ISI
SICI code
0169-328X(20010713)91:1-2<169:RHMO1A>2.0.ZU;2-#
Abstract
Acetylcholine is the main neurotransmitter of the vestibular efferents and a wide variety of muscarinic and nicotinic acetylcholine receptors are expr essed in the vestibular periphery. To date, 11 nicotinic subunits (alpha an d beta) have been reported in mammals. Previously, our group [Brain Res. 77 8 (1997) 409] reported that there nicotinic acetylcholine receptor alpha an d beta subunits were differentially expressed in the vestibular periphery o f the rat. To begin an understanding of the molecular genetics of these ves tibular efferents, this study examined the chromosomal locations of these n icotinic acetylicholine receptor genes in the rat (Rattus norvegicus). Usin g radiation hybrid mapping and a rat radiation hybrid map server (www.rgd.m cw.edu/RHMAP SERVER/), we determined the chromosomal position for each of t hese genes. The alpha2-7, alpha9, alpha 10, and beta2-4 nicotinic subunits mapped to the following chromosomes: alpha2, chr. 15; alpha3, chr. 8; alpha 4, chr. 3; alpha5, chr. 8; alpha6, chr. 16; alpha7, chr. 1: alpha9, chr. 14 ; alpha 10, chr. 7; beta2, chr. 2; beta3, chr. 16: and beta4, chr. 8. With the location for each of these nicotinic subunits known, it is now possible to develop consomic and/or congenic strains of rats that can be used to st udy the functional genomics of each of these subunits. (C) 2001 Elsevier Sc ience B.V. All rights reserved.