M-CSF transgenic mice: Role of M-CSF in infection and autoimmunity

Citation
T. Bernier et al., M-CSF transgenic mice: Role of M-CSF in infection and autoimmunity, EXP TOX PAT, 53(2-3), 2001, pp. 165-173
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
ISSN journal
0940-2993 → ACNP
Volume
53
Issue
2-3
Year of publication
2001
Pages
165 - 173
Database
ISI
SICI code
0940-2993(200106)53:2-3<165:MTMROM>2.0.ZU;2-R
Abstract
In this study, transgenic CD2F1 mouse lines (C-1.1-C-1.11) bearing a transg ene encoding the murine growth factor M-CSF under the control of the liver specific alpha-1-antitrypsin gene promoter were generated. Transgenic C-1.4 mice showed elevated expression of transgene-encoded M-CSF in the liver an d displayed a 2-3-fold increase of M-CSF plasma levels and of macrophage nu mbers in the liver as compared with non-transgenic littermates. M-CSF trans genic mice showed increased resistance against sublethal i.v. infections wi th Listeria monocytogenes as compared with infected non-transgenic mice. To investigate the influence of M-CSF in murine systemic lupus erythematosu s (SLE), the M-CSF transgenic mouse line C-1.4 was bred into the genetic ba ckground of SLE-prone MRL+/+ mice. The resulting C-1.4/MRL transgenic mice bearing increased endogenous M-CSF levels showed consistently lower levels of anti-ss-DNA autoantibodies as compared with non-transgenic MRL+/+ mice. The life span of the C-1.4/MRL transgenic mice and the severity of the dise ase in these mice remained unchanged as compared with their non-transgenic littermates. It is concluded that in addition to M-CSF further factors must be involved in the acceleration of the autoimmune disease in SLE prone MRL /1pr mice.