Heterochromatin and interstitial telomeric DNA homology

Citation
As. Multani et al., Heterochromatin and interstitial telomeric DNA homology, CHROMOSOMA, 110(3), 2001, pp. 214-220
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CHROMOSOMA
ISSN journal
0009-5915 → ACNP
Volume
110
Issue
3
Year of publication
2001
Pages
214 - 220
Database
ISI
SICI code
0009-5915(200107)110:3<214:HAITDH>2.0.ZU;2-N
Abstract
The purpose of this investigation was twofold. The first objective was to d emonstrate that, in most of ten mammalian species commonly used in biomedic al research, not all constitutive heterochromatin (C-bands) represents telo meric DNA. For example, the C-bands in human chromosomes, the long arm of t he X and the entire Y chromosome of Chinese hamster, and most of the short arms of Peromyscus and Syrian hamster chromosomes are not telomeric DNA. In addition to the usual terminal telomeric DNA in the chromosomes of these m ammalian species, the pericentromeric regions of seven or eight Syrian hams ter chromosomes and all Chinese hamster chromosomes except pair one have pe ricentromeric regions that hybridize with telomeric DNA, some in C-bands an d some not. The second objective was to describe a simple fluorescence in s itu hybridization (FISH) reverse-printing procedure to produce black-andwhi te microphotographs of metaphase and interphase cells showing locations of telomeric DNA with no loss of resolution. Thus, at least three different ty pes of heterochromatin (telomeric heterochromatin, nontelomeric heterochrom atin and a combination of both) are present in these mammalian species, and this simple black-andwhite reverse printing of telomeric FISH preparations can depict them economically without sacrificing clarity.