Selectivity tuning and molecular modeling of new generation packings for RP HPLC

Citation
B. Buszewski et al., Selectivity tuning and molecular modeling of new generation packings for RP HPLC, CHROMATOGR, 53, 2001, pp. S204-S212
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
0009-5893 → ACNP
Volume
53
Year of publication
2001
Part
2
Supplement
S
Pages
S204 - S212
Database
ISI
SICI code
0009-5893(2001)53:<S204:STAMMO>2.0.ZU;2-T
Abstract
Retention mechanism, selectivity and physicochemical properties together wi th theoretical surface simulation experiments of laboratory-synthesized pac king materials for high performance liquid chromatography (HPLC) containing cholesterol molecules immobilized on the high quality silica gel were stud ied. The chromatographic behavior of the three cholesterol-based columns (S G-CHOL, SG-MIX CHOL/AP and SG-10-CHOL) was examined with special regard to retention and selectivity and compared to reference materials: a typical oc tadecylsilica column (SG-C18) and an alkylamide bonded phase (SG-AP). Tempe rature investigations (DSC) and molecular modeling simulations didn't bring an expected confirmation of liquid crystalline properties of materials stu died. The observed phase transition was attributed to conformation change w ithin the amide bonding.