Determination of urinary pyrraline by solid-phase extraction and high performance liquid chromatography

Citation
K. Yoshihara et al., Determination of urinary pyrraline by solid-phase extraction and high performance liquid chromatography, BIOL PHAR B, 24(8), 2001, pp. 863-866
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
8
Year of publication
2001
Pages
863 - 866
Database
ISI
SICI code
0918-6158(200108)24:8<863:DOUPBS>2.0.ZU;2-H
Abstract
Pyrraline is one of the advanced glycation end products formed under non-en zymatic and non-oxidative conditions in vivo. In this study, we developed a novel method for determination of urinary pyrraline using solid-phase extr action as a pretreatment procedure prior to determination by high performan ce liquid chromatography (HPLC). The Oasis(TM) HLB solid-phase extraction c artridge was used for pretreatment of urine samples without hydrolysis. The chromatogram obtained clearly revealed the peak for urinary pyrraline owin g to prior removal of interfering substances in urine samples. The recovery rate of pyrraline was 97.2+/-3.3% (n=6). The mean excretion level of urina ry pyrraline in healthy control (20-77 years old, n=30) was 1.42+/-0.65 mu mol/mmol creatinine, and the daily variation in the excretion level was con sidered to be insignificant. We propose the above procedure as a simple, ra pid, and accurate method for determination of pyrraline levels in urine.