Identification of three new splice variants of the SNARE protein SNAP-23

Citation
A. Shukla et al., Identification of three new splice variants of the SNARE protein SNAP-23, BIOC BIOP R, 285(2), 2001, pp. 320-327
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
285
Issue
2
Year of publication
2001
Pages
320 - 327
Database
ISI
SICI code
0006-291X(20010713)285:2<320:IOTNSV>2.0.ZU;2-5
Abstract
SNAP-23 has an important role in protein-trafficking processes in mammalian cells and until yet two isoforms of SNAP-23 (SNAP-23a and SNAP-23b) have b een described. In the present report, we have identified the existence of t hree new SNAP-23 isoforms (named SNAP-23c, SNAP-23d, and SNAP-23e), which a rise from alternative splicing. By RT-PCR all five splice variants were sho wn to be expressed in four different human inflammatory cells (eosinophils, basophils, neutrophils, and peripheral blood mononuclear cells). Transfect ion of the human basophilic KU-812 cell line with plasmid constructs contai ning the cDNAs of the five splice variants located SNAP-23a and SNAP-23b pr imarily in the plasma membrane. The other three splice variants were locali zed both intracellularly and in the plasma membrane. (C) 2001 Academic Pres s.