Immunolocalization of the angiogenetic factor pleiotrophin (PTN) in the growth plate of mice

Citation
W. Petersen et M. Rafii, Immunolocalization of the angiogenetic factor pleiotrophin (PTN) in the growth plate of mice, ARCH ORTHOP, 121(7), 2001, pp. 414-416
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Ortopedics, Rehabilitation & Sport Medicine
Journal title
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
ISSN journal
0936-8051 → ACNP
Volume
121
Issue
7
Year of publication
2001
Pages
414 - 416
Database
ISI
SICI code
0936-8051(200107)121:7<414:IOTAFP>2.0.ZU;2-7
Abstract
The aim of this study was to find out whether and where the angiogenic agen t pleiotrophin (PTN) occurs within the growth plate. We investigated paraff in-embedded tissue sections of ten male mice with an antibody directed agai nst the recombinant PTN. Immunostaining for PTN was positive within the cyt oplasm and the pericellular matrix of osteoblasts which lined the longitudi nal mineralized septae of the epiphyseal plate. Within the zone of hypertro phic chondrocytes, immunolabelling for PTN was positive in the pericellular matrix of hypertrophic chondrocytes and within the opened lacunae of the a poptotic hypertrophic chondrocytes. The resting zone and the proliferation zone were PTN negative. The results of our study suggest that the known ang iogenetic peptide PTN plays a role in the process of angiogenesis in the gr owth plate.