Expression of adhesion molecules and fibronectin of activated peritoneal surface with Lipopolysaccharide (LPS) analyzed with Immuno SEM

Citation
Y. Liang et al., Expression of adhesion molecules and fibronectin of activated peritoneal surface with Lipopolysaccharide (LPS) analyzed with Immuno SEM, ANN ANATOMY, 183(4), 2001, pp. 353-356
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
ISSN journal
0940-9602 → ACNP
Volume
183
Issue
4
Year of publication
2001
Pages
353 - 356
Database
ISI
SICI code
0940-9602(200107)183:4<353:EOAMAF>2.0.ZU;2-P
Abstract
To disclose cell-to-cell interaction associated with the defensive mechanis m of the peritoneum, the peritoneum was stimulated with lipopolysaccharide (LPS) and analyzed three-dimensionally, ultrastructurally, and immunohistoc hemically with immunoSEM (scanning electron microscopy). The activated hepa tic peritoneal surface demonstrated numerous microvilli with the adhesion m olecules ICAM-1 and VCAM-1. They were restricted to villi and peaked at 1.5 mug/g body weight of LPS. Delicate strands appeared moderately and were in terwoven among microvilli with increasing LPS. These strands did not expres s ICAM-1 or VCAM-1, but fibronectin. Leukocytes began to adhere to the peri toneal surface above die value of LPS (2.5 mug). These results suggest that the peritoneal surface gives a defensive sheet for cell-to-cell interactio n through adhesion molecules and fibronectin.