Sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato

Citation
K. Maeo et al., Sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato, PLANT MOL B, 46(5), 2001, pp. 627-637
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT MOLECULAR BIOLOGY
ISSN journal
0167-4412 → ACNP
Volume
46
Issue
5
Year of publication
2001
Pages
627 - 637
Database
ISI
SICI code
0167-4412(2001)46:5<627:SEITPO>2.0.ZU;2-S
Abstract
Expression of genes coding for sporamin and beta -amylase, the two most abu ndant proteins in storage roots of sweet potato, is coordinately inducible in atypical vegetative tissues by sugars. A sweet potato gene for beta -amy lase (beta -Amy) with introns as well as a beta -Amy::GUS fusion gene compo sed of the beta -Amy promoter and the GUS coding sequence, both showed suga r-inducible expression in leaves of transgenic tobacco which occurred via a hexokinase-independent pathway. Analyses using various 5'-terminal and int ernal deletions of the beta -Amy promoter indicated that truncated promoter s of beta -Amy containing a sequence between -901 and -820, relative to the transcription start site, and the basic promoter region can confer sugar-i nducible expression. This 82 bp region contained the TGGACGG sequence that plays an essential role in the sugar-inducible expression of the truncated promoter of the sporamin gene. Deletion or base substitutions of this eleme nt in the truncated beta -Amy promoter abolished the sugar-inducible expres sion, the results suggesting that the TGGACGG element plays an important ro le in the coordinate induction of expression of genes for beta -amylase and sporamin by sugars.