APETALA1 and SEPALLATA3 interact to promote flower development

Citation
S. Pelaz et al., APETALA1 and SEPALLATA3 interact to promote flower development, PLANT J, 26(4), 2001, pp. 385-394
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
26
Issue
4
Year of publication
2001
Pages
385 - 394
Database
ISI
SICI code
0960-7412(200105)26:4<385:AASITP>2.0.ZU;2-4
Abstract
In Arabidopsis, the closely related APETALA1 (APT) and CAULIFLOWER (CAL) MA DS-box genes share overlapping roles in promoting flower meristem identity. Later in flower development, the AP1 gene is required for normal developme nt of sepals and petals. Studies of MADS-domain proteins in diverse species have shown that they often function as heterodimers or in larger ternary c omplexes, suggesting that additional proteins may interact with AP1 and CAL during flower development. To identify proteins that may interact with AP1 and CAL, we used the yeast two-hybrid assay. Among the five MADS-box genes identified in this screen, the SEPALLATA3 (SEP3) gene was chosen for furth er study. Mutations in the SEP3 gene, as well as SEP3 antisense plants that have a reduction in SEP3 RNA, display phenotypes that closely resemble int ermediate alleles of APT. Furthermore, the early flowering phenotype of pla nts constitutively expressing AP1 is significantly enhanced by constitutive SEP3 expression. Taken together, these studies suggest that SEP3 interacts with AP1 to promote normal flower development.