Frequency of HPRT mutants in humans exposed to vinyl chloride via an environmental accident

Citation
R. Becker et al., Frequency of HPRT mutants in humans exposed to vinyl chloride via an environmental accident, MUT RES-GTE, 494(1-2), 2001, pp. 87-96
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
ISSN journal
1383-5718 → ACNP
Volume
494
Issue
1-2
Year of publication
2001
Pages
87 - 96
Database
ISI
SICI code
1383-5718(20010725)494:1-2<87:FOHMIH>2.0.ZU;2-H
Abstract
The mutant frequency (MF) in the hypoxanthine-guanine-phosphoribosyl-tranfe rase (HPRT) locus of peripheral blood T-lymphocytes was measured in a popul ation environmentally exposed to vinyl chloride - a toxic and carcinogenic substance through an accidental release into the atmosphere. It was compare d to MF in a control group of unexposed individuals. Both groups were re-in vestigated in a follow-up study, 2 years later. No significant difference c ould be observed in MF between exposed and controls either at the accident nor in the follow-up study. Approximately the same mean HPRT mutant frequen cies were observed for both groups in T-lymphocytes from blood samples obta ined shortly after the accident and from the follow-up blood samples, Both groups showed a higher mean MF in the re-investigation samples which is mos t probably due to the significantly lower average cloning efficiency (CE) u nder non-selective conditions and because of the inverse relationship betwe en CE and ME The exposed population showed a higher mean T-cell CE at the i nitial blood sampling as compared to the control group. The concurrent cyto genetic analyses of peripheral lymphocytes showed a significant increase in cells with aberrations in the exposed population. Clastogenic but not muta genic activity of vinyl chloride was observed in our study. (C) 2001 Elsevi er Science B.V. All rights reserved.