In vitro transcription/DNA synthesis using isolated organelle-nuclei: Application to the analysis of the mechanisms that regulate organelle genome function

Authors
Citation
A. Sakai, In vitro transcription/DNA synthesis using isolated organelle-nuclei: Application to the analysis of the mechanisms that regulate organelle genome function, J PLANT RES, 114(1114), 2001, pp. 199-211
Citations number
56
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF PLANT RESEARCH
ISSN journal
0918-9440 → ACNP
Volume
114
Issue
1114
Year of publication
2001
Pages
199 - 211
Database
ISI
SICI code
0918-9440(200106)114:1114<199:IVTSUI>2.0.ZU;2-H
Abstract
Plant cells contain two distinct types of DNA-containing organelles, plasti ds and mitochondria, which are both considered to be descendants of endosym bionts. DNAs of these organelles associate with specific proteins to form c ompactly organized DNA-protein complexes, referred to as organelle-nuclei ( or nucleoids), in which various functions of organelle genomes, such as rep lication and gene expression (transcription), are performed. An assay of in vitro transcription/DNA synthesis using isolated organelle-nuclei provided us with a suitable system for analysis of the mechanisms that regulate the functions of organelle genomes. In this review, I focus on (1) the concept of organelle-nuclei, (2) construction and characterization of an in vitro transcription/DNA synthesis system using isolated organelle-nuclei, (3) ana lysis of the transcriptional regulation of plastid gene expression with emp hasis on the presence of distinct RNA polymerase activities in the plastids , and (4) analysis of the DNA synthesizing apparatus of the plastids and mi tochondria, considering the possible interaction during evolution between t he organelles.