Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests

Citation
T. Marrodan et al., Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests, J MED MICRO, 50(8), 2001, pp. 663-666
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF MEDICAL MICROBIOLOGY
ISSN journal
0022-2615 → ACNP
Volume
50
Issue
8
Year of publication
2001
Pages
663 - 666
Database
ISI
SICI code
0022-2615(200108)50:8<663:EOTMTM>2.0.ZU;2-F
Abstract
The results of a dipstick assay for the detection of immunoglobulin M (IgM) to Brucella smooth lipopolysaccharide (S-LPS) correlated with those of an enzyme-linked immunosorbent assay (ELISA) for IgM and of the serum agglutin ation test (SAT) performed with and without dithiothreitol, Two sera which were dithiothreitol-sensitive and were dipstick negative were shown to cont ain specific IgA, The dipstick assay is recommended as a simple method for detecting specific IgM antibodies in acute-phase brucellosis patients.