Dendritic cells derived from patients with multiple sclerosis show high CD1a and low CD86 expression

Citation
Ym. Huang et al., Dendritic cells derived from patients with multiple sclerosis show high CD1a and low CD86 expression, MULT SCLER, 7(2), 2001, pp. 95-99
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurology,"Neurosciences & Behavoir
Journal title
MULTIPLE SCLEROSIS
ISSN journal
1352-4585 → ACNP
Volume
7
Issue
2
Year of publication
2001
Pages
95 - 99
Database
ISI
SICI code
1352-4585(200104)7:2<95:DCDFPW>2.0.ZU;2-N
Abstract
Dendritic cells (DC) ore important antigen presenting cells (APC) and play a major role in initiating and orchestrating immune responses by priming T cells. Little is known about involvement of DC in multiple sclerosis (MS), where auto-aggressive T cells against myelin autoantigens ore considered to contribute to inflammation and demyelination in the central nervous system . In this study, we compared phenotype and cytokine secretion of DC from pa tients with MS, other neurological diseases (OND) and healthy subjects. DC were generated from blood adherent mononuclear cells (MNC) by culture for 7 days with granulocyte-macrophage colony-stimulating factor (GM-CSF) and in terleukin-4 (IL-4). The yield and morphology of DC were similar in MS patie nts and controls. In both, the DC phenotype was that of immature myeloid li neage, comprising CD1a(+) and CD1c(+). The proportion of CD1a(+) DC, being important for presentation of lipid antigens to T cells, was higher in MS p atients compared to controls. The proportion of CD86(+) DC, a co-stimulator y molecule that is assumed to promote Th2 differentiation, was low in MS. L ow proportions of CD86(+) DC were only observed in untreated MS Patients bu t not in patients treated with IFN-P. Production of IL-10 and IL-12 p40 by DC did not differ in MS patients and controls. These Findings indicate that alterations of functionally important surface molecules on DC ore associat ed with MS.