Bladder transplantation in rats using FK-506

Citation
A. Yamataka et al., Bladder transplantation in rats using FK-506, J UROL, 166(1), 2001, pp. 259-262
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
JOURNAL OF UROLOGY
ISSN journal
0022-5347 → ACNP
Volume
166
Issue
1
Year of publication
2001
Pages
259 - 262
Database
ISI
SICI code
0022-5347(200107)166:1<259:BTIRUF>2.0.ZU;2-F
Abstract
Purpose: We created viable bladder tissue by transplantation with immunosup pression. Materials and Methods: For bladder transplantation the bladder of newborn B rown-Norway rats was excised and each was transplanted into a pouch created in the distal omentum of a 5-week-old Lewis rat. In 15 group 1 rats no imm unosuppressive agent was used. In 20 group 2 rats 0.6 mg./kg. FK-506 daily were given intramuscularly until a predetermined day of harvest. Recipient rats were sacrificed on day 3, 5, 7 or 14 after bladder transplantation, an d the bladder grafts were harvested and formalin fixed. Hematoxylin and eos in staining was done to examine bladder graft survival and the degree of re jection, and immunohistochemical testing was performed for assessing the ve sical nervous system. In 5 rats in the control group bladder augmentation w as performed by anastomosing the bladder graft to the native bladder. Each augmented bladder was harvested 21 days later for histopathological assessm ent. Results: Overall bladder graft survival was 96.4%. Each successfully transp lanted bladder graft appeared macroscopically as a thin walled cyst. In gro up 1 all bladder grafts showed rejection with cellular infiltration. In gro up 2 there was mild rejection in 5 rats and no evidence of rejection in the remaining 15. All group 2 bladder grafts had intact nerve distribution. Bl adder augmentation was successful in all 5 cases and the mucosa was normal throughout each augmented bladder. Conclusion: Because FK-506 successfully prevents rejection, our technique w ould appear to have the potential for creating viable bladder tissue that m ay be used for bladder augmentation in cases of vesical exstrophy or neurog enic bladder.