Bupropion hydrochloride: The development of a chiral separation using a chiral AGP column

Citation
J. Munro et al., Bupropion hydrochloride: The development of a chiral separation using a chiral AGP column, J LIQ CHR R, 24(3), 2001, pp. 327-339
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
3
Year of publication
2001
Pages
327 - 339
Database
ISI
SICI code
1082-6076(2001)24:3<327:BHTDOA>2.0.ZU;2-9
Abstract
A chiral high-performance liquid chromatographic separation was developed f or bupropion active ingredient. The mobile phase parameters that may influe nce the separation were studied and included: mobile phase pH, type and con centration of organic modifier, type and concentration of buffer, amount of analyte injected, and column temperature. The optimized chiral separation baseline resolved the two enantiomers in less than 10 minutes. Method validation was also performed for the separation. The method was fou nd to be linear over a range of 0.5 mug/g to 99.8 mug/g with a correlation coefficient greater than 0.999. The limit of detection was determined to be 0.25 mug/g while the limit of quantitation was found to be 0.5 mug/g. Method precision was found to be 0.8% and 1.9% for the two enantiomers, whi le the system precision was found to be 0.4% and 0.5% for the two enantiome rs.