Calculation of phase residence times in micellar electrokinetic chromatography

Citation
M. Idei et al., Calculation of phase residence times in micellar electrokinetic chromatography, J LIQ CHR R, 24(3), 2001, pp. 303-315
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
3
Year of publication
2001
Pages
303 - 315
Database
ISI
SICI code
1082-6076(2001)24:3<303:COPRTI>2.0.ZU;2-4
Abstract
Method to calculate micellar phase residence time [t(mic) = t(mc) k''', tim e spent by the analyte in the micellar phase] and aqueous phase residence t ime [t(aq) = t(0) (1 - k"'), time spent by the analyte in the aqueous phase ] was developed, where t(m), t(o) and t(mc) are the migration time of the a nalyte, the flow marker and the micelles, respectively. It was proved that migration time of the analyte is the sum of the phase residence times [t(m) = t(mic) + t(aq) = t(0) (1 - k"') + k"' t(mc)]. Two physico-chemical param eters characterizing hydrophobicity of the analyte, namely the ratio of the moles of the analyte in the micellar (n(mc)) and aqueous (n(aq)) phase (n( mc)/n(aq) = t(mi)c/t(aq)) as well as distribution coefficient (K) have also been determined from the phase residence times. Good correlation was found between the micellar phase residence times and computer calculated hydroph obicity values (S) in the case of hydroxy- and aldehyde benzene derivatives , benzene- and alkyl phenone homolog series and in the case of seven hydrop hobic protected peptides. Comparison of hydrophobicity values and phase res idence times showed that the higher the hydrophobicity of the analyte the l onger the time spent in the micellar (hydrophobic) phase. The micellar phas e residence time determinable experimentally in MEKC reflects the hydrophob icity of the molecule investigated.