Assay of-flavone aglycones in extracts of Origanum vulgare from Macedonia i
ncluding identification and isolation is performed. Hydrolyzed extracts wer
e analyzed by thin layer chromatography (TLC) and high performance liquid c
hromatography (HPLC). Three aglycones were identified and then isolated by
column chromatography and TLC. Further TLC, HPLC, and UV/VIS spectrophotome
tric analysis showed that the isolated compounds are luteolin (the most abu
ndant), apigenin, and diosmetin.
A new and rapid HPLC method was developed only for determination of the con
tent of luteolin, which can serve as an appropriate criterion for evaluatio
n of the flavonoid content in the drug. The accuracy of the method was prov
en by the method of standard additions, which gave satisfactory values for
the recovery (98.2-100.3 %). The quantity of luteolin found in the drug ran
ged from 0.40-0.50 % (m/m).