Identification, isolation, and determination of flavones in Origanum vulgare from Macedonian flora

Citation
S. Kulevanova et al., Identification, isolation, and determination of flavones in Origanum vulgare from Macedonian flora, J LIQ CHR R, 24(4), 2001, pp. 589-600
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
4
Year of publication
2001
Pages
589 - 600
Database
ISI
SICI code
1082-6076(2001)24:4<589:IIADOF>2.0.ZU;2-H
Abstract
Assay of-flavone aglycones in extracts of Origanum vulgare from Macedonia i ncluding identification and isolation is performed. Hydrolyzed extracts wer e analyzed by thin layer chromatography (TLC) and high performance liquid c hromatography (HPLC). Three aglycones were identified and then isolated by column chromatography and TLC. Further TLC, HPLC, and UV/VIS spectrophotome tric analysis showed that the isolated compounds are luteolin (the most abu ndant), apigenin, and diosmetin. A new and rapid HPLC method was developed only for determination of the con tent of luteolin, which can serve as an appropriate criterion for evaluatio n of the flavonoid content in the drug. The accuracy of the method was prov en by the method of standard additions, which gave satisfactory values for the recovery (98.2-100.3 %). The quantity of luteolin found in the drug ran ged from 0.40-0.50 % (m/m).