Identification and determination of the active compounds in Gastrodia elata Blume by HPLC

Citation
Hx. Li et al., Identification and determination of the active compounds in Gastrodia elata Blume by HPLC, J LIQ CHR R, 24(4), 2001, pp. 579-588
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
4
Year of publication
2001
Pages
579 - 588
Database
ISI
SICI code
1082-6076(2001)24:4<579:IADOTA>2.0.ZU;2-I
Abstract
A simple, sensitive, and specific high-performance liquid chromatography me thod has been developed for the simultaneous quantification of the three ma jor biologically active ingredients, gastrodin (GA), 4-hydroxybenzyl alcoho l (HA), and 4-hydroxybenzaldehyde (HD), in Gastrodia elata Blume for the fi rst time. The HPLC assay was performed on a reversed phase ODS column by us ing methanol-water-isopropyl alcohol (35:55:10, v:v:v) as mobile phase with a flow rate of 0.4mL/min. The detection wavelength was set at 270 nm. Regr ession equations revealed linear relationships (correlation coefficients: 0 .9997-0.9999) between the peak area of each constituent (GA, HA, HD) and it s concentration. The relative standard deviations of the retention times of three constituen ts range between 0.2 similar to0.5%. The recoveries for the three constitue nts ranged between 96.6 similar to 98.5%. The GA, HA, HD contents measured 12.0 mg/g (2.53% RSD), 2.1 mg/g (2.04% RSD), and 0.13 mg/g (2.63% RSD), res pectively, in the ethanol extracts of Gastrodia elata Blume.