The application of a practical approach for the optimization of the separat
ion of a mixture of triazine and substituted urea herbicides and a mixture
of organophosphorous insecticides is presented. The separation is achieved
with reversed-phase HPLC and acetonitrile/water mobile phase mixtures. The
separation method is based on the simultaneous optimization of temperature
(T) and gradient steepness (b) as a means of varying selectivity and achiev
ing an acceptable resolution.
The effect of flow rate is also examined. Triazine and substituted urea her
bicides exhibit greater changes in resolution than organophosphorous insect
icides with variation in T and b. This is attributed to the more diverse an
d polar molecular nature of the herbicide mixture. Flow rate is used as an
additional parameter that could facilitate resolution. The overall separati
on is achieved within a few experiments.