The HPLC separation of two mixtures of neutral pesticides: Effects of temperature, gradient steepness, and flow rate

Citation
A. Kotrikla et Td. Lekkas, The HPLC separation of two mixtures of neutral pesticides: Effects of temperature, gradient steepness, and flow rate, J LIQ CHR R, 24(4), 2001, pp. 543-553
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
4
Year of publication
2001
Pages
543 - 553
Database
ISI
SICI code
1082-6076(2001)24:4<543:THSOTM>2.0.ZU;2-O
Abstract
The application of a practical approach for the optimization of the separat ion of a mixture of triazine and substituted urea herbicides and a mixture of organophosphorous insecticides is presented. The separation is achieved with reversed-phase HPLC and acetonitrile/water mobile phase mixtures. The separation method is based on the simultaneous optimization of temperature (T) and gradient steepness (b) as a means of varying selectivity and achiev ing an acceptable resolution. The effect of flow rate is also examined. Triazine and substituted urea her bicides exhibit greater changes in resolution than organophosphorous insect icides with variation in T and b. This is attributed to the more diverse an d polar molecular nature of the herbicide mixture. Flow rate is used as an additional parameter that could facilitate resolution. The overall separati on is achieved within a few experiments.