Reverse phase HPLC method for determination of vancomycin in influenza vaccine

Citation
P. Forlay-frick et al., Reverse phase HPLC method for determination of vancomycin in influenza vaccine, J LIQ CHR R, 24(4), 2001, pp. 497-507
Citations number
3
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
24
Issue
4
Year of publication
2001
Pages
497 - 507
Database
ISI
SICI code
1082-6076(2001)24:4<497:RPHMFD>2.0.ZU;2-5
Abstract
A simple and sensitive high-performance liquid chromatographic method for t he determination of vancomycin in influenza-vaccine has been developed. The stationary phase was a Purospher RP-18e column (125 x 3.5 mm; 5 mum; Merck ); the mobile phase consisted of acetonitrile-water-phosphoric acid (85%) ( 8.5 / 91.5 / 0.125 v/v/v %) and its pH was adjusted to pH 2.80 using distil lated triethylamine immediately prior to use. Separation was achieved using a flow rate of 0.5 mL/min at ambient temperat ure. The vancomycin was detected at 230 nm. The retention time for vancomyc in was 7.20 +/- 0.20 min. The limit of detection was 30 ng/mL; the limit of quantification was found to be 100 ng/mL.