A simple and sensitive high-performance liquid chromatographic method for t
he determination of vancomycin in influenza-vaccine has been developed. The
stationary phase was a Purospher RP-18e column (125 x 3.5 mm; 5 mum; Merck
); the mobile phase consisted of acetonitrile-water-phosphoric acid (85%) (
8.5 / 91.5 / 0.125 v/v/v %) and its pH was adjusted to pH 2.80 using distil
lated triethylamine immediately prior to use.
Separation was achieved using a flow rate of 0.5 mL/min at ambient temperat
ure. The vancomycin was detected at 230 nm. The retention time for vancomyc
in was 7.20 +/- 0.20 min. The limit of detection was 30 ng/mL; the limit of
quantification was found to be 100 ng/mL.